It is important to contact a healthcare provider if any of these side effects persist or worsen
144 Morevoer, Zhao et al
Animals and diets Male Wistar rats ( n = 42) purchased from the Veterinary Sciences Institute of Litoral (Instituto de Ciencias Veterinarias del Litoral, ICIVET-Litoral) -Facultad de Veterinaria, Universidad Nacional del Litoral (Esperanza, Santa Fe, Argentina) were maintained with unrestricted access to water and food under controlled temperature (22 1 C), humidity and air flow conditions, with a fixed 12-h light/dark cycle (light on 07.00 a.m to 7.00 p.m)
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Experiment Condiitons Cell Line: HeLa cells Pretreatment: DMEM (cystine-free, serum-free), 37, 5 min Uptake conditions0.5 mmol/l sulfasalazine or 2 mol/l erastin / Cystine Analog / DMEM (cystine-free, serum-free), 37, 30 min Instrument: Fluorescent Plate Reader Filter: Ex=485 nm, Em=535 nm Application Data 2: Comparison with Radio Isotope Method Cystine uptake assay, which used to require RI measurement, can now be easily measured with a plate reader