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Reverse transcription of miRNA was performed using the Hairpin-it miRNAs qPCR Quantitation Kit (GenePharma, Shanghai, China), following the manufacturers protocol

Upon ligand binding, TR II phosphorylates and activates TR I, forming a heteromeric receptor complex that initiates downstream signaling through both SMAD-dependent and SMAD-independent pathways, such as ERK, p38, JNK, and PI3K-Akt cascades [33, 36, 39]

D.FrisoniG.et al (2017)

The at-home version trades that consistency for convenience and cost

Additionally, intravenous administration of the endogenous peptide apelin-13 can significantly reduce BBB permeability and vasogenic edema by targeting oxidative stress during ischemia-reperfusion