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Kitzbichler MG, Aruldass AR, Barker GJ, Wood TC, Dowell NG, Hurley SA, et al

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Peptides were extracted from each sample using HyperSep C18 tips (Thermo Scientific), and 50 g and 350 g were kept for total proteomics and phosphoproteomics analysis, respectively

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Thrombin was removed by Benzamidine-Sepharose 6B resin (Cytiva) and tag-free TbTR was further purified by reverse immobilized metal affinity chromatography (Protino Ni-TED resin, Macherey-Nagel)