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Protection of proteins from oxidative stress: a new illusion or a novel strategy

Giacconi R, Muti E, Malavolta M, Cipriano C, Costarelli L, Bernardini G, et al

BLI binding assays were performed in BLI buffer (20 mM TrisHCl, pH 8.0, 150 mM NaCl, 15 mM MgCl 2 , 0.02% LMNG, and 0.1% BSA) and assay mixtures were prepared in black 96-well plates with wells filled to 200 L

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5 mM) in all mammalian cells and carries out a wide range of functions including acting as a coenzyme for glutathione reductase in the recycling of dehydroascorbic acid into ascorbic acid