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Organotypic hippocampal slice cultures (OHSC) Mice (P6-8) were decapitated, brains were aseptically removed, and hippocampi were dissected under sterile conditions in ice-cold isolation buffer (pH 7.2) containing Gey's balanced salt solution (Thermo Fisher Scientific, Dreieich, Germany, #J67569.K2), 1 mM kynurenic acid (a NMDA receptor antagonist

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The study was conducted in a mouse model with BRAF and NRAS mutations, as well as wild-type melanoma xenografts and allografts of highly metastatic melanoma (222)