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Acute effect of high-dose isoflavones from Pueraria lobata (Willd.) Ohwi on lipid and bone metabolism in ovariectomized mice

2.8 Functional cell assays moDCs were stained with the fixable viability dye eFluor 780 (eBiosciences, San Diego, CA, USA) for 20 min, followed by staining with anti-HLA-DR APC (clone G46-6, BD Biosciences), anti-CD83 FITC (clone HB15e, BD Biosciences), anti-CD86 PE-CF594 (clone 2331, BD Biosciences), anti-CD1c PerCP-eFluor 710 (clone L161, Thermo Fisher Scientific), and anti-CD45 BV510 (Clone HI30, BD Biosciences)