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The review confirmed that animal and human studies demonstrate adequate protein nutrition is crucial for maintenance of GSH homeostasis, and that enteral or parenteral cystine, methionine, N-acetyl-cysteine, and L-2-oxothiazolidine-4-carboxylate are effective cysteine precursors for tissue GSH synthesis

Biological evaluations included both in vitro and in vivo tests

doi: 10.3168/jds.S0022-0302(02)74397-X 267 ReedsP

RNA analysis Tissue samples were crushed on dry ice and homogenized using a bead Mill (VWR)

To collect pollen from bees, various types of pollen-collecting traps are designed (with bee size small holes) and transplanted with the hive entrance gates for the purpose of detaching pollen from the legs of bees when they enter the hive (190, 191)