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The following primary antibodies and respective dilutions were used: BRN2 (Novus Biologicals, NBP2-21585) 1:400

The quality of log-linear regression and, consequently, the reliability of the extrapolated pharmacokinetic parameters (namely z , t , AUC 0 , AUC extra , V z , CL r and CL t ) was demonstrated by a determination coefficient, R 2 0.8

The HMB remaining available in the rumen, in turn, exhibits comparable behavior to unaltered HMB (Noftsger et al., 2005)

BPC-157: Works locally at injection site Fast pain relief (3-7 days) Best for acute injuries Inject near injury More immediate results TB-500: Works systemically throughout body Slower initial results (7-14 days) Best for chronic injuries Inject anywhere Better long-term tissue quality For most injuries, start with BPC-157