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To determine cell viability, 3,000 HT-1080 or MDA-MB-231 cells were seeded in a 96-well plate with standard medium (10% FBS and 1% penicillinstreptomycin) around 20 h before treatment

A single administration of 8 at 42 d.p.i (200 mg/kg i.p.) resulted in a 19% and 7% decrease in worm and egg burdens, respectively

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Notably, addition of hemin or pyruvate, a source for NAD+ did not impact these growth phenotypes, suggesting that amelioration of heme defect or respiration inhibition is not sufficient to overcome anti-proliferative effects of mitochondrial GSH depletion (Extended Data Fig