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All other cysteine residues could be identified in its reduced form and the average amount of free thiols (-SH) was 90% in the water treated samples ( Figure 6A )

Single-particle image processing and 3D reconstruction All ME2-EA or ME2-MDSA image stacks acquired in counting mode were imported into Relion for motion correction and dose weighting using MotionCor2 46 with a 5 5 patch without binning (resulting in a pixel size of 0.82 /pixel)