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Molecular mechanisms of radioactive iodine refractoriness in differentiated thyroid cancer: Impaired sodium iodide symporter (NIS) expression owing to altered signaling pathway activity and intracellular localization of NIS

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Post-RbCl treatment, cells were washed, incubated with 10 M DCFH-DA at 37 C for 30 min in the dark, washed again, trypsinized, and resuspended in phosphate-buffered saline (PBS)