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After 30-45 minutes, you may leave it on or wash with water

Methods Cell culture KBM7 inducible Cas9 (iCas9) cells (gift from Johannes Zuber / IMP - Research Institute of Molecular Pathology) were cultured in IMDM (Gibco, 21980032) and 293T cells (ATCC, CRL-3216) or Lenti-X 293T (Clontech, 632180) in DMEM (Gibco, 41965062), both supplemented with 100 U ml 1 penicillin/streptomycin (Sigma-Aldrich, P4333) and 10% fetal calf serum (FCS, Gibco, A5256701), unless specified otherwise

In some embodiments, the nanoparticles comprise other lipoprotein-based nanoparticles, such as HDL, HDL-like lipoprotein particles or synthetic HDL-like particles (see, e.g., U.S