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From the 1950s to the 1980s, cerebellar stimulation gained more attention (albeit controversial) for its use in the treatment of disease [156, 394,395,396]

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The bound protein was eluted using binding buffer supplemented with 150 mM imidazole at a flow rate of 3 ml/min and applied onto the HiTrap SP FF 5-ml column (pre-equilibrated with 20 mM sodium phosphate, pH 6.0, with 50 mM NaCl) at a flow rate of 1 ml/min

Herpes simplex virus type 1 classically causes a severe, necrotizing encephalitis localizing to the temporal lobes, presenting with altered mental status and seizures

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