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10.1038/s41598-022-05078-1 283

Enzyme turnover assays were performed at 37C in 96 well clear bottom plates (Genesee Scientific, #25-104) in a Synergy HTX plate reader in 200L PBS using 200M NADPH, 0.2M enzyme and a range of 4-HNE concentrations from 0 to 0.4mM