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Transduction efficiency (~3070%) was measured 3 days after transduction by flow cytometry (BECKMAN COULTER Model: CytoFLEX S) in a 96-well plate (Corning Incorporated (Costar) Ref: 3897), followed by measuring the viability of transduced cells after 6, 9, 13, and 15 days

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15 Similarly, thermo-responsive liposomes (Asp/TLs), with a size of ~114 nm and 84% encapsulation efficiency, selectively released aspirin under mild hyperthermia (40C), leading to a fourfold increase in cytotoxicity against triple-negative breast cancer (MDA-MB-231)