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RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) We isolated RNA from zebrafish skeletal muscle using the NucleoSpin RNA Midi kit (MACHEREYNAGEL, US), and performed reverse transcription-quantitative PCR (RT-qPCR) according to the protocol as described in our paper 28

The PLAG1-GDH1 axis promotes anoikis resistance and tumor metastasis through CamKK2-AMPK signaling in LKB1-deficient lung cancer

References to food supplements have not been evaluated by the EFSA (European Food Safety Authority) and are not intended to diagnose, treat, cure, or prevent any disease