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doi: 10.1016/j.apcatb.2011.04.019

has received grant funding and/or honoraria from: the NIH (grants P30 DK089507, R01HL103965, R01AI101307 and UM1HL119073), Food and Drug Administration (grant R01FD003704), the Cystic Fibrosis Foundation, Vertex Pharmaceuticals, Transave Inc., L

Twenty-four hours later, approximately 80% confluent cells were co-transfected with pCS2+8-NLS-Halo and Lipofectamine 3000 as per the manufacturers recommendations

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The standard protocol involves 5 to 10 mg daily via subcutaneous injection for 10 to 20 consecutive days, repeated once or twice per year with 4 to 6 months between cycles