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RNA isolation and qPCR analysis Total RNA from skin and lungs was isolated by RNeasy Fibrous tissue mini kits (Qiagen, Germantown, MD, 74,704)

[DIAGRAM: Two identical glasses of water one full, one at 90% due to dehydration with the same drop of alcohol dye added to each, showing visibly higher concentration in the smaller volume] How the Widmark r-Factor Connects to Hydration Scientific explanation: The Widmark Formula the standard equation used to estimate BAC uses a constant called the r-factor (approximately 0.73 for men and 0.66 for women) that represents the proportion of body weight available as water for alcohol to distribute into

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