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The centrifugation that is routinely used in order to separate RBCs from the other components of source whole blood, and to remove cell suspension buffers following application of pharmacological probes used to test mechanistic hypotheses, can itself deform the cells and elicit further, artifactual ATP release

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To obtain the specific labeling patterns, intracellular metabolites were extracted and analyzed using GC/MS to determine multiple ion detections (MIDs) (corrected for natural isotope abundance), which reflect relative metabolic fluxes 28

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