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All reagents are detailed in Supplemental Table 1

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The core function of the physical barrier of intestinal epithelium The function of the physical barrier of intestinal epithelium can be summarized as two core functions: "selective permeability" and "defensive protection", which work together to maintain the homeostasis of the intestinal environment

By avoiding the limitations of oral intake such as low bioavailability and digestive breakdown

Compared with the DM group, GI group had 13 differential metabolites, including urea, glycerol, glycine, -D-mannose, cadaverin, L-proline, glutamine, L-phenylalanine, L-asparagine, D-glucose, D-gluconic acid, indole and cholesterol (Figure 2F)