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Name: glutathione nerve sheath mylene Myelin Myelin Sheath: What It Is,
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It is found up-regulated in CKD with renal fibrosis and is significantly elevated in early stages of AKI

The WHO defined a biomarker as any substance, structure, or process that can be measured in the body or its products and influence or predict the incidence of outcome or disease (WHO 2001)

11 , e1004841 (2015)

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected