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Figure 9 shows FACS analysis of M25 IgGl binding to live HEK293 cells transfected with ALPPL2-expressing plasmid, along with a plasmid expressing the green fluorescence protein (GFP)

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Benitez A, Fieremans E, Jensen JH, Falangola MF, Tabesh A, Ferris SH, Helpern JA (2014) White matter tract integrity metrics reflect the vulnerability of late-myelinating tracts in Alzheimer's disease

(2021) used intra-articular injection and decide to replicate it

We therefore wondered whether lack of Thr or Cys would also trigger similar generic AA starvation responses, even though the final fate of deprivation of either AA was a distinct form of cell death