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Description

Carbamoyl phosphate, produced by carbamoyl phosphate synthetase 1 (CPS1), which is located in the mitochondria, together with ornithine is used to generate citrulline

The following sgRNAs for GGT1 CRISPRCas9 KO were used: sgGGT1 1 (HL-ID: 6) forward: 5-CACCGTGCGGGACGGTGGCTCTG-3

Moreover, in accordance with our request to obtain a library with an equal content of each type of nucleotide, NGS analysis revealed a minor bias in the nucleotide distribution