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Weight of evidence: Overall evidence is LowModerate in VPA models

GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)

It may be that there are general dietary (precursor amino acids and purines are both high in many foods), hydration, hepatic, or other influences that affect purines and indoleamines and TYR similarly

Coffier M, Marion-Letellier R, Dchelotte P

Similar yields and purities were obtained when the recombinant proteins were produced for 4 h at 30 C in E