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Description

The complexed sample was then purified using a 5 mL Hitrap desalting column (Cytiva) with buffer C (50 mM HEPES, pH = 8, 150 mM NaCl, 10% (v/v) glycerol, 5 mM BME, 2 mM MgCl 2 and 0.03% (w/v) DDM)