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The isolation of mitragynine, speciociliatine, speciogynine, paynantheine, corynantheidine, and ajmalicine were conducted using a procedure described previously

ATP levels were then normalized to cell number

While d-peptides can be readily made by SPPS, d-protein binder synthesis requires the same tedious process consisting of SPPS, NCL of peptides and subsequent refolding as production of the d-target protein for screening