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10.1111/j.1471-4159.2012.07734.x J

around defect (duodenum, colon), local blood vessels failure versus rapid particular vascular effect)

The next day, the membranes were washed three times with TBST and then incubated with anti-rabbit IgG conjugated with IRDye 800CW (LI-COR Biosciences, Lincoln, Nebraska, USA) for 1 h at RT

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RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)