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After ultrasonic lysis, the supernatant samples were divided into IP, IgG, and Input, where Input was preserved at -20 and the other two were incubated overnight with the antibodies H3K27ac (5 l, CST, #8173) and TFAP2A (5 l, Affinit, AF5323)

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To worsen the scenario, TNF also decreased production of things like nitric oxide, a powerful blood vessel dilator that could help the kidneys recover, as well as prostaglandin E2, which can suppress the immune response and inflammation