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PMID: 42285368 Biochem Pharmacol5-Methoxyseselin inhibits neuronal ferroptosis and -amyloid production in female APP/PS1 transgenic mice

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doi: 10.1073/pnas.1000092107 266 WenZ.HeM.PengC.RaoY.LiJ.LiZ.et al

The following reported sgRNAs and shRNAs were used in this study: LYSET sgRNA1: 5 TGTCCACACCCAAAAAGGCA 3, GNPTAB sgRNA1: 5 ACTCATTGCGATCTATCGAG 3, GNPTAB sgRNA2 (KI): 5 CTTCTATACTCTGATTCGAT 3, VPS35 sgRNA1: 5 CTCAAGGGATGTTGCACACC 3 GOLPH3 sgRNA1: 5 AGGGCGACTCCAAGGAAACG 3, GOLPH3L sgRNA2: 5 GGATATCCGCCTTACTCTTA 3, GOLPH3 shRNA1: 5 GAGAAACAGAACTTCCTACTT 3 Sample preparation and western blotting Cells were collected in ice-cold 1X PBS, pelleted at 2700 g for 1 min, and lysed in lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% Triton) containing protease inhibitor cocktail (Bimake) at 4 C for 20 min

Functional significance of mitochondrial bound hexokinase in tumor cell metabolism