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The protein was then centrifuged at 110,000 g for 1 h at 4 C using an ultracentrifuge (Sorvall M120, Discovery, Thermo Fisher Scientific, UK) and stored, at the concentration 30 mg/ml, at 20 C under argon until used

2.5.5 Measurement of CAT activity Aebis method was applied, in which CAT activity is determined using a spectrophotometric method based on the breakdown of hydrogen peroxide by catalase (33)

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This natural distribution system ensures that all tissues get adequate glutathione protection, even if they cant make much themselves