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A 400 L aliquot of 4% RBCs was mixed with 400 L of SeMSN or RBC@SeMSN@Uri at various concentrations (12.5, 25, 50, 100, and 200 gmL 1 ) and incubated at 37C for 24 h

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This concentration balances solubility with practical application volumes higher concentrations (above 5mg/mL) risk incomplete dissolution and peptide aggregation, while lower concentrations require larger application volumes that dilute the effective dose per surface area