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After centrifugation for 10 min at 4 C, the supernatants were decanted, and the protein pellet was prepared for immunoblotting with G6PD antibody

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The reactions were complemented by adding the Tb-conjugated anti-Flag antibody (Cisbio, 61FG2TLF) at 15 pg l 1 in a final total volume of 11 l and incubations were carried out for additional 10 min at 20 C