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Exosomes were also validated in terms of size, shape, and quality by using Transmission Electron Microscopy, following a standard protocol as described previously 32 (JEOL 2000EXII TEM, Neuroscience Institute, The University of Tennessee Health Science Center)

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Instead of producing ATP, these dysfunctional mitochondria generate excessive amounts of reactive oxygen species (ROS)

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Before loading the sample on the column, the stationary phase must be equilibrated with the high ionic strength buffer (the same buffer that the protein sample will be applied in)