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After pulse-vortexing, the mixture was transferred to BACTEC mycobacteria growth indicator tubes (MGITs) with PANTA (polymyxin-B, amphotericin-B, nalidixic acid, trimethoprim, azilocillin) antibiotics and Oleic Albumin Dextrose Catalase (OADC) enrichment broth (Becton Dickinson, UK) until positivity was reached and expressed as TTD

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