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Primary antibodies for western blotting Immunoblotting was performed with rabbit polyclonal anti-GCL (1:1,000) 5 , rabbit polyclonal anti-GPX1 (1:1,000) (ab59546, Abcam, Cambridge, UK), rabbit polyclonal anti-GFP (1:1,000) (ab290, Abcam), mouse monoclonal anti-GSS (1:1,000) (Sc-166882, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-GSR (Sc-133159, Santa Cruz Biotechnologies), mouse monoclonal anti-SOD2 (1:2,000) (ab16,956, Abcam), rabbit polyclonal anti-VDAC (1:1,000) (Calbiochem, Darmstadt, Germany) or mouse monoclonal anti-GAPDH (1:40,000) (glyceraldehyde-3-phosphate dehydrogenase

doi: 10.1016/j.plaphy.2023.108263 137 XuL.YueQ.XiangG.BianF.YaoY

Moreover, sevoflurane post-treatment could preserve NAD+ levels and inhibit mPTP opening in the aging myocardium under stress by increasing the expressions of PI3K and ERK1/2 pathways [107]

During this process, 5-Amino-1MQ may preserve lean muscle mass

When the level of these hormones is too low, the balance of chemical reactions in the body is upset and important functions are impacted