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Nox2-derived radicals contribute to neurovascular and behavioral dysfunction in mice overexpressing the amyloid precursor protein

Invitrogen/Thermo Fisher Scientific) was employed to generate cDNA and to carry a linear co-amplification (1114 cycles) of three amplicons: two derived from target genes (final concentration of each primer was 80 nM) and one derived from the RPL8 reference gene (final concentration of each primer was 60 nM)