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Patients must receive folic acid, vitamin B12, and corticosteroids before and during treatment to mitigate certain side effects

Axons that touch the dashed lines and are totally inside the unbiased counting frame were counted

To quantify the expression of S-100, GDNF, and BDNF in transplanted human GMSCs, the area of colocalized immunolabeling signals (in yellow-orange pixels) of these proteins (in green color) and human nuclei (in red color) in the merged files was measured using ImageJ program and presented as the percentage of colocalization = the area of yellow-orange pixels/total area of green pixels [42]

NeuroRehabilitation 2005;20:183-90

Then, we continue for one more hour to the starting point