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Further analysis of the scRNA-seq data revealed additional gene expression alterations that support our previous findings (Fig

Anchoring interactions with AGO2 MID ZSWIM8 residue F1261 docks at the centre of the MID-domain interface, and its mutation to alanine reduced AGO2 polyubiquitylation in vitro and abrogated TDMD in cells (Fig

Nowak L, et al

The compatibility of His-tag with denaturing agents can be a benefit for recombinant proteins produced as inclusion bodies, as they require steps of solubilization with chaotropic agents prior to binding to the column (Hochuli et al., 1988)

Previous studies have discovered that drugs could be delivered into brain via the vesicular transport, a vectorial movement across the cerebral endothelial cells from the luminal to the abluminal side 6