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Liao Y, Zeng T, Guo X, Li X

The sections were then incubated in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 1% Triton-X, .05% Tween-20, and .05% sodium azide) at room temperature for 1 h and incubated at 4C overnight with the following primary antibodies

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Prior to experimentation, each rat received 6 training sessions on 3 consecutive days, and animals that retained for 5 min on the rod were nominated to do the test