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Building on our previous published work, we employed an immunopeptidomic approach to identify surface-presented mycobacterial antigens following infection of THP-macrophages with BCG or MTB ( in silico -based approach to analyze the repertoire of MHC-bound peptides present in each identified antigen, allowing us to optimize candidate antigen selection based on epitope richness

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The endo-lysosomal pathway is a major bottleneck limiting the clinical efficacy of therapeutic agents, as nanoparticles are often sequestered and degraded following endocytosis, reducing bioavailability ( in vitro , as evidenced by the dispersion of gene cargo around the nucleus and a transient peak in the colocalization coefficient followed by a decrease, indicating successful endosomal escape