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Total CD8 + T cells were purified from PBMCs by magnetic-activated cell sorting using CD8 microbeads (Miltenyi, 130-045-201)

Similar to what is observed in monocytes and macrophages, G6PD-d may limit the production of NADPH-dependent ROS that act as second messengers to enhance NFAT, AP-1, and NFB signaling 89,116,117

Here, 12-week-old male wild-type mice (C57BL/6 J) were treated with a 0.3% CPZ diet for 3 weeks

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