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From Day 10, neuronal maturation media, consisting of DMEM-F12/Glutamax, N2 supplement, B27 supplement, BDNF (20 ng/ml), Lam (1 g/ml), GDNF (20 ng/ml, Peprotech 450-10), dibutyryl-cAMP (500 g/ml, Selleckchem S7858), ascorbic acid (200 nM, Sigma A4544), AraC (2 M), and P/S (1X) was used with half media changes every 23 days

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