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The cells were mechanically crushed by using a cell scraper in 2 mL of methanol after six times PBS washing (2 mL each)

This study was very kindly funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) DFG-FOR2886 PANDORA Project-No.1, DFG-CRC1181-Project-No.B07

Since Vitamin B12 is found in meat, fish, eggs and dairy products mainly, vegans are most at risk of developing deficiency associated with diet, but its easily fixed with tablets

Older Adults : The ability to absorb Vitamin B12 decreases with age, partly due to reduced stomach acid production

High B12 is not usually dangerous on its own, but may point to issues like liver disease, kidney dysfunction, or blood cancers