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The integrated approach demonstrated in this paper opens a new window to better studied the H 2 O 2 generation and variation in complete cell culture medium in a straightforward and rapid manner, which can greatly help scientists to investigate H 2 O 2 related biomedical phenomena

The cell-free supernatant was combined with a sulfhydryl preserving solution and assayed for glutathione content by HPLC and normalized based on the urea dilution method as described previously ( STATISTICS Nominal data are presented as means with SE and analyzed by ANOVA and Dunnets or Bennetts post hoc test as indicated

PTMs necessitate more investigations to concurrently address target specificity and global metabolic consequences, which simultaneously unveil novel scientific perspectives for personalized therapeutic strategies