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Similarly, in callus cultured on media containing Si-based materials, PPO activity was higher in the presence of 8 mg L -1 nano form (9.760.08 nM min -1 g -1 FW) and lower in bulk form at 4 mg L -1 (8.410.08 nM min -1 g -1 FW)

Culture media (DMEM/Ham F12 with HEPES and 4.5 g/L glucose), Dulbeccos phosphate-buffered saline (Dulbeccos PBS), Versene, antibiotics, and fetal calf serum (FCS) were purchased from Invitrogen

To delay this degenerative progression of the disease, the role of GDNF is reported (120), possibly contributing to the increase of dendrites length, number of dendrites, or a combination of both

As the leading cause of chronic joint pain and functional disability, OA affects over 595 million individuals worldwide, with incidence rates escalating with aging populations and obesity epidemics [3, 4]