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4 Clinical testing and assessment related to ferroptosis 4.1 Biomarkers 4.1.1 Definition and mechanism of ferroptosis biomarkers Ferroptosis, as a novel form of programmed cell death, involves complex mechanisms and multiple biomolecules and metabolic pathways (54, 55)

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Therefore, to explore the link between TNF- and the ROS-dependent mode of cell death, the experimental groups were examined by flow cytometry, which revealed that TPA caused a marked increase in ROS, whereas 200 ng/ml TNF- significantly reversed the increase in ROS (Fig

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