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Sterility is readily accomplished by filtration through sterile filtration membranes (e.g., 0.2 micron membranes)

The following siRNA duplex was used for silencing Tfeb expression: siRNA sense: 5 GGAUCAAGGAGCUGGGAAUUU 3, antisense: 5 AUUCCCAGCUCCUUGAUCCUU 3 (Dharmacon)

Si bien normalmente son el resultado del estrs, los radicales libres pueden ser causados por otros factores ambientales como el humo y los contaminantes