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Dual luciferase reporter assay For the luciferase assay, 293 T cells were cultured in 24-well plates at 70 to 80% confluency and co-transfected with the plasmids encoding Ppara -wild, Ppara -mut, Pparg -wild, or Ppard- wild, along with the PPRE plasmid and a control vector (Renilla luciferase), using Lipofectamine 3000 Reagent (Invitrogen, L3000150) according to the manufacturers protocols

Unknown factors include potential hormonal effects, cellular signaling imbalance, and unintended tissue growth responses after prolonged exposure

GABA is the primary inhibitory neurotransmitter in the mammalian brain where it acts predominantly through GABA A Rs

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