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Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum

* Correspondence: Yan Cui, [email protected]

Additionally, the distribution of male and female patients was relatively balanced, with male patients comprising 42.90% and female patients 49.30%, revealing no statistically significant differences (refer to Supplementary Tables S8,S9)

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