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Apoptosis assay (flow cytometry) The apoptosis rate of Raji cells was determined by flow cytometry using a dead cell apoptosis kit with Annexin V Alexa Fluor 488 and propidium iodide (Invitrogen)

Gallagher JEG, Ser SL, Ayers MC et al (2020) The polymorphic PolyQ tail protein of the mediator Complex, Med15, regulates the variable response to diverse stresses

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After TMB substrate solution is added, only those wells that contain Human CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color